DNA,RNA, Recombinant DNA Technology

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DNA,RNA, Recombinant DNA Technology

1: DNA,RNA, Recombinant DNA Technology

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18: Gene expression.

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38: X-Ray structure of a complex of ethidium bromid with DNA.

39: Construction of a restriction map.

40: Restriction map for the 5243-bp circular DNA of SV40.

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43: Construction of a recombinant DNA molecule through the use of synthetic oligonucleotide adaptors

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47: Insertional inactivation Gene in cloning site: LacZ - pUC18 (lacZ complements the host defect in lacZ) - pUC18 into host organism - active lacZ (β-galactosidase) from plasmid- cleavage of X-gal (blue colonies) - gene cloned into polylinker - lacZ gene disrupted - no cleavage of X-gal (white colonies)

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49: Insertional inactivation Gene in cloning site: Resistance marker - pBR322 (cloning sites within antibiotica resistence marker) - plasmid into host - resistance against 2 antibiotica - gene cloned within one resistance marker - gene for antibiotica resistance marker disrupted - sensitive against one antibioticum

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53: Electron micrograph of bacteriophage λ.

54: Bacteriophage T2 injecting its DNA into an E. coli

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63: Cloning of foreign DNA in λ phages.

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65: Creation of Libraries

66: Creation of Libraries

67: Sizes of Some DNA Molecules.

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73: Fragmentation of genomic DNA

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83: Bacterial host engineering

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85: E. Coli K12 strain has been used for further engineering

86: Additional changes in K12 E. coli for ease of the laboratory practice

87: Additional changes in K12 E. coli for ease of the laboratory practice

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89: Transformation of plasmid DNA to competent E. coli cells

90: Calcium/phosphate (heat shock) method

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